Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene HIV-1 genotyping assay.

@article{Hallack2008EvaluationOD,
  title={Evaluation of dried blood spot specimens for HIV-1 drug-resistance testing using the Trugene HIV-1 genotyping assay.},
  author={Renee Hallack and Lauren E Doherty and Judith A Wethers and Monica M. Parker},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2008},
  volume={41 4},
  pages={283-7}
}
BACKGROUND Efforts to simplify the collection and shipping of specimens for HIV drug-resistance testing in resource-limited settings are needed as antiretroviral therapy increases worldwide. OBJECTIVE To evaluate the reliability and practicality of using dried blood spots (DBS) for HIV-1 drug-resistance testing with the Trugene HIV-1 genotyping assay. STUDY DESIGN Nucleic acids from 33 DBS and counterpart plasma specimens were extracted using the Nuclisens MiniMAG system and genotyped using… CONTINUE READING
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