Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis.

@article{Saxena2003EvaluationOD,
  title={Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray analysis.},
  author={Alka Saxena and Elizabeth A. Worthey and Shaofeng Yan and Aaron Leland and Kenneth D. Stuart and Peter J. Myler},
  journal={Molecular and biochemical parasitology},
  year={2003},
  volume={129 1},
  pages={103-14}
}
The various Leishmania species are flagellated protozoans, responsible for a wide spectrum of human diseases. The sequence of the L. major genome is nearing completion and a large proportion of the identified genes have yet to be ascribed functions. DNA microarrays containing PCR-amplified DNA from a random amplified genomic library of L. major Friedlin (LmjF) [Mol. Biochem. Parasitol. 113 (2001) 337] were hybridized with fluorescent probes made from L. major Friedlin RNA from five time-points… CONTINUE READING