Evaluation of different methods detecting intracellular generation of free radicals

@article{Wilhelm2009EvaluationOD,
  title={Evaluation of different methods detecting intracellular generation of free radicals},
  author={Jiř{\'i} Wilhelm and Richard Vyt{\'a}{\vs}ek and Ivana O{\vs}ť{\'a}dalov{\'a} and Luděk Vajner},
  journal={Molecular and Cellular Biochemistry},
  year={2009},
  volume={328},
  pages={167-176}
}
Reactive oxygen species (ROS) play several biological roles. We investigated the applicability of fluorescent probes for their detection (i) in rabbit lens epithelial cells during ageing in culture, and (ii) in thin sections of rat heart. We used dihydroethidium (DHE), dichlorofluorescin (DCFH), and dihydrorhodamine 123 (DHR) together with detection of autofluorescence both in cells and in chloroform extracts. Superoxide production was confirmed by a specific histochemical method using Mn2… CONTINUE READING

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