Evaluation of different DNA extraction procedures for the detection of Salmonella from chicken products by polymerase chain reaction.

@article{Soumet1994EvaluationOD,
  title={Evaluation of different DNA extraction procedures for the detection of Salmonella from chicken products by polymerase chain reaction.},
  author={Christophe Soumet and Gwennola Ermel and Patrick Fach and Pierre Colin},
  journal={Letters in applied microbiology},
  year={1994},
  volume={19 5},
  pages={294-8}
}
Polymerase chain reaction (PCR) was used after a short pre-enrichment culture to detect Salmonella subspecies in chicken fillets. A direct PCR assay performed with chicken meat inoculated with Salmonella Typhimurium produced no PCR products. Six different DNA extraction protocols were tested to recover efficiently Salmonella DNA after a short incubation period. Three of them gave results that were reliable, rapid and sensitive. Successful protocols used Proteinase K and/or a centrifugation step… CONTINUE READING