Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.

@article{Maher2003EvaluationOC,
  title={Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.},
  author={Majella Maher and Cathriona Finnegan and Evelyn Collins and Brid Ward and Cyril Carroll and Martin Cormican},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 7},
  pages={2980-6}
}
Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in modified Preston broth, with or without blood added, for 48 h at 37 degrees C prior to plating. A 16S/23S PCR/DNA probe membrane-based colorimetric detection assay was evaluated on… CONTINUE READING