Evaluation of cranial window types for in vivo two-photon imaging of brain microstructures.

@article{Isshiki2014EvaluationOC,
  title={Evaluation of cranial window types for in vivo two-photon imaging of brain microstructures.},
  author={Masaaki Isshiki and Shigeo Okabe},
  journal={Microscopy},
  year={2014},
  volume={63 1},
  pages={53-63}
}
In vivo two-photon laser scanning microscopy (2PLSM) of neurons and glia in the mouse neocortex can be achieved using two distinct surgical methods: the thin-skull and open-skull window techniques. The open-skull window technique is more invasive, but provides more flexibility in imaging periods and frequencies. Critical evaluations of the two surgical methods are required, particularly their ability to image submicron structures such as dendritic filopodia and spines. Here, we used multiple… CONTINUE READING
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