Evaluation of confocal laser scanning microscopy for enumeration of virus-like particles in aquatic systems

@article{Peduzzi2012EvaluationOC,
  title={Evaluation of confocal laser scanning microscopy for enumeration of virus-like particles in aquatic systems},
  author={Peter Peduzzi and M Papadopoulos Agis and Birgit Luef},
  journal={Environmental Monitoring and Assessment},
  year={2012},
  volume={185},
  pages={5411-5418}
}
Abundances of virus-like particles (VLPs, mostly bacteriophages) are high in aquatic environments; therefore, techniques for precise enumeration are essential in ecological monitoring. VLPs were determined after staining with SYBR Gold by conventional epifluorescence microscopy and compared to enumerations performed by confocal laser scanning microscopy (CLSM). In order to assess the potential of CLSM for viral direct counts (VDCs), we processed samples from different freshwater and marine… CONTINUE READING

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A comparison of methods for counting viruses in aquatic systems.

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