Evaluation of colorimetric loop-mediated isothermal amplification assay for visual detection of Streptococcus agalactiae and Streptococcus iniae in tilapia.

@article{Suebsing2013EvaluationOC,
  title={Evaluation of colorimetric loop-mediated isothermal amplification assay for visual detection of Streptococcus agalactiae and Streptococcus iniae in tilapia.},
  author={Rungkarn Suebsing and Jantana Kampeera and B Tookdee and Boonsirm Withyachumnarnkul and Wes Turner and Wansika Kiatpathomchai},
  journal={Letters in applied microbiology},
  year={2013},
  volume={57 4},
  pages={317-24}
}
UNLABELLED Streptococcus agalactiae and Strep. iniae are bacterial pathogens that cause streptococcosis in many fish species. An accelerated colorimetric loop-mediated isothermal amplification (LAMP) assay with pre-addition of calcein was established, and the transmission and detection of Strep. agalactiae and Strep. iniae in tilapia under natural aquatic environment were investigated. A positive reaction was observed by a colour change from orange to green through the naked eyes after… CONTINUE READING