Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR

@article{Cruz2009EvaluationOC,
  title={Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR},
  author={Fernanda L. Cruz and Samara Kalaoun and Paula Macedo Nobile and Carlos Eduardo Dias Colombo and Juliana Ferreira de Almeida and Leila Maria Gomes Barros and Eduardo A A Romano and Maria Fatima Grossi-de-Sa and Mait{\'e} F. S. Vaslin and M{\'a}rcio Alves-Ferreira},
  journal={Molecular Breeding},
  year={2009},
  volume={23},
  pages={607-616}
}
Accuracy in quantitative real-time polymerase chain reaction (qPCR) requires the use of stable endogenous controls. Normalization with multiple reference genes is the gold standard, but their identification is a laborious task, especially in species with limited sequence information. Coffee (Coffea ssp.) is an important agricultural commodity and, due to its economic relevance, is the subject of increasing research in genetics and biotechnology, in which gene expression analysis is one of the… CONTINUE READING

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Chlorogenic acid synthesis in coffee : an analysis of CGA content and real - time RT - PCR expression of HCT , HQT , C 3 H 1 , and CCoAOMT 1 genes during grain development in C . canephora

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