Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitus.

@article{inkilic2009EvaluationOC,
  title={Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitus.},
  author={Nilufer Çinkilic and Sinem Kıyıcı and Serap Çelikler and Ozgur Vatan and Ozen Oz Gul and Ercan Tuncel and Rahmi Bilaloğlu},
  journal={Mutation research},
  year={2009},
  volume={676 1-2},
  pages={
          1-4
        }
}
Oxidative stress-induced DNA damage seems to play a role in the pathogenesis of type-1 diabetes mellitus and its complications. Several in vitro assays have been used to measure the DNA damage produced by oxidative stress. In the present study, we aimed to investigate the frequency of sister chromatid exchange (SCE), chromosomal aberrations (CA) and micronuclei (MN) in type-1 diabetes mellitus patients compared with healthy controls. SCE, CA and MN tests were carried out with the blood-cell… Expand
Evaluation of the frequency of sister chromatid exchanges and micronuclei in children with type-1 diabetes mellitus
TLDR
An increased level of SCE and MN can be a common cytogenetic marker in children with T1DM, and SCE may be a more sensitive early parameter for genomic instability. Expand
Association of elevated frequency of micronuclei in peripheral blood lymphocytes of type 2 diabetes patients with nephropathy complications.
TLDR
The results indicate that increased genomic instability expressed as MNs is associated with nephropathy in all pathological stages and implementation of MN assay in clinical level may potentially enhance the quality of management of patients with diabetes and its complications such as nephtropathy. Expand
Evaluation of DNA damage in Type 2 diabetes mellitus patients with and without peripheral neuropathy: A study in South Indian population
TLDR
The use of the Cytokinesis Block Micronucleus Cytome assay as a potent tool for the identification of DNA damage, which may prove to be useful biomarker to assess the severity diabetes-associated complications such as neuropathy, is highlighted. Expand
Elevated micronuclei frequency in type 2 diabetes with high glycosylated hemoglobin.
TLDR
The increased glycosylation seems to induce oxidative damage in the DNA of the diabetic patients, which manifests as an increased micronuclei frequency, which has a potential to be used as a biomarker for subsequent diabetic complications. Expand
Nuclear abnormalities in buccal mucosa cells of patients with type I and II diabetes treated with folic acid.
TLDR
FA supplementation in patients with DM reduced the frequency of NAs and the significantly reduced frequencies of buccal mucosa cells with micronuclei, binucleation, pyknosis, karyorrhexis, kARYorr hexis+abnormally condensed chromatin, kolyolysis, and nuclear buds produced by FA supplementation in DM patients are consistent with the idea that free radicals are responsible for the increased frequency ofNAs inDM patients. Expand
Chromosomal damage measured by the cytokinesis block micronucleus cytome assay in diabetes and obesity - A systematic review and meta-analysis.
TLDR
A clear trend towards increased genome damage in these metabolic diseases was observed and type 1 and type 2 diabetics, disease progression as well as medical quality and quantity were linked to further elevated genome instability. Expand
Apoptosis and genome instability in children with autoimmune diseases
TLDR
Children with AITD (HT and GD) and T1DM have a significantly lower level of apoptosis in PBLs and significantly higher MN frequency as GDI than healthy subjects and Apoptosis and the level of genome instability in these patients with AID are positively correlated. Expand
Genotoxic Effect in Autoimmune Diseases Evaluated by the Micronucleus Test Assay: Our Experience and Literature Review
TLDR
A review of work and other published papers concerning genotoxic effect in AD, identified by means of the MN assay is presented, with the aim of proposing this tool as a possible early biomarker for genot toxic damage, which is a consequence of disease progression. Expand
Cytogenetic and Immunogenic Study of Type -2 Diabetes Mellitus Patients
TLDR
The study results showed that chromosomal state effected significantly in the DM patients by reducing both of mitotic index and blastogenic index while the chromosomal aberrations increased gradually according to the glucose level in b lood and age. Expand
Micronucleus Analysis in Behçet ’ s Disease With and Without HLA-B 51 HLA-B 51
Objective: Genetic factors are considered to play important roles in the development of Behçet’s disease (BD). Micronuleus (MN) assay is a sensitive indicator of caused genomic damage. Our aim was toExpand
...
1
2
3
4
...

References

SHOWING 1-10 OF 28 REFERENCES
Increased frequency of sister chromatid exchanges in patients with type II diabetes
TLDR
Increased frequency of SCE in diabetic patients could be an early marker of DNA damage and a predisposing factor for some diabetes-related complications, and there is a need to study other markers ofDNA damage in DM. Expand
Assessment of DNA strand breakage by comet assay in diabetic patients and the role of antioxidant supplementation.
TLDR
Clear differences between patients on placebo and on Vitamin E supplementation for 12 weeks were observed on the basis of the extend of DNA migration during single cell gel electrophoresis, which might indicate that IDDM patients are handling more oxidative damage on a regular basis. Expand
Chromosome aberrations, micronuclei, aneuploidy, sister chromatid exchanges, and cancer risk assessment.
TLDR
The aim of this paper is to encourage cytogeneticists to design their experiments in such a way that the data obtained will be of maximum possible benefit for characterizing and quantifying adverse human health effects, particularly cancer. Expand
An examination of DNA strand breakage in the comet assay and antioxidant capacity in diabetic patients.
There are two forms of diabetes, insulin-dependent Diabetes mellitus (IDDM) and non-insulin dependent Diabetes mellitus (NIDDM). There is evidence to suggest that reactive oxygen is involved in theExpand
Superoxide dismutase activity and chromosome damage in cultured chromosome instability syndrome cells.
TLDR
The observed parallelism between the SOD activity and the cytogenetic manifestation may imply an involvement of active oxygen species, especially superoxide radicals, in the increased chromosome damage of CIS cells. Expand
DNA damage and antioxidant defense in peripheral leukocytes of patients with Type I diabetes mellitus.
TLDR
Impaired antioxidant defense in leukocytes of patients with Type 1 DM may be one of the responsible mechanisms for increased DNA damage in those patients. Expand
Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes.
TLDR
Data indicate that decreased GSH level may be a contributory factor for enhanced oxidative DNA damage in type 2 diabetics; and chronic hyperglycemia derived from poorly-controlled diabetic conditions may induce oxidativeDNA damage in these patients. Expand
Measurement of micronuclei by cytokinesis-block method in bovine lymphocytes.
TLDR
The results demonstrate that the optimal concentration of cytochalasin B is needed to obtain the highest frequency of binucleated cells in bovine lymphocytes, which is 3 times higher than that reported in human lymphocytes. Expand
Mitotic recombination is an important mutational event following oxidative damage.
TLDR
The results suggest that the biologically important consequence of some ROS may be LOH as a by-product of recombination repair, and that if the observations apply to ROS generally, then many of the mutations which accumulate with ageing or which are observed in cancer may be due to factors other than ROS. Expand
Persistent oxidative stress in chromosomally unstable cells.
TLDR
The data suggest that the perpetuation of many of the abnormal phenotypes associated with genomic instability may be linked to a state of chronic oxidative stress derived in part from dysfunctional mitochondria. Expand
...
1
2
3
...