Evaluation of chromogenic substrates for measurement of protease production by biocontrol strains of Trichoderma.

Abstract

Four chromogenic substrates were compared, and methods were developed for measuring protease activity from fungi. Digestion of azoalbumin, a water-soluble substrate, resulted in dye release most closely proportional to enzyme activity. Substrates insoluble in water were advantageous for time-course studies, and azocoll was more sensitive to digestion and… (More)

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