Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA.

@article{Muskhelishvili2003EvaluationOC,
  title={Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA.},
  author={Levan Muskhelishvili and John R. Latendresse and Ralph L. Kodell and Eric Henderson},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2003},
  volume={51 12},
  pages={1681-8}
}
The standard method for assessment of cell proliferation in paraffin-embedded tissue sections is 5-bromodeoxyuridine (BrdU) immunohistochemistry (IHC). BrdU can be administered to laboratory animals via IP injections, is readily incorporated into nuclei during the DNA synthetic phase of the cell cycle, and is detected with an anti-BrdU antibody. This method has several disadvantages, and an accurate method for evaluation of proliferative activity that can substitute for BrdU IHC, when necessary… CONTINUE READING

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