Evaluation of canine lymphocyte proliferation: comparison of three different colorimetric methods with the 3H-thymidine incorporation assay.

@article{Wagner1999EvaluationOC,
  title={Evaluation of canine lymphocyte proliferation: comparison of three different colorimetric methods with the 3H-thymidine incorporation assay.},
  author={Ulrike Gabriella Wagner and Eberhard Burkhardt and Klaus Failing},
  journal={Veterinary immunology and immunopathology},
  year={1999},
  volume={70 3-4},
  pages={151-9}
}
To evaluate canine lymphocyte stimulation the radioactive thymidine incorporation assay is still the method of choice. In order to find a suitable non-radioactive alternative to the standard 3H-thymidine incorporation assay, proliferation of canine peripheral blood lymphocytes (PBL) was measured with three different colorimetric assays, using the two tetrazolium salts MTT and XTT and 5-bromo-deoxyuridine (BrdU). Isolated canine PBL were stimulated with two different mitogens, Concanavalin A… CONTINUE READING