Evaluation of biological responses to polymeric biomaterials by RT-PCR analysis. I. Study of IL-1 beta mRNA expression.

@article{Kishida1996EvaluationOB,
  title={Evaluation of biological responses to polymeric biomaterials by RT-PCR analysis. I. Study of IL-1 beta mRNA expression.},
  author={Akio Kishida and Shinzi Kato and Kayo Ohmura and Kazuro Sugimura and Mitsuru Akashi},
  journal={Biomaterials},
  year={1996},
  volume={17 13},
  pages={1301-5}
}
In this study, we introduce a novel research methodology, the evaluation of mRNA expression of cells contacting with polymeric materials using reverse transcription-polymerase chain reaction (RT-PCR) analysis HL-60 was used as a model of the macrophages. The expression of interleukin-1 beta (IL-1 beta) mRNA, a cytokine secreted by macrophages, was selected to estimate the extent of inflammation. The expression of IL-1 beta mRNA in the HL60 cells cultured on various substrates and in various… CONTINUE READING