Evaluation of approaches to generation of tissue-specific knock-in mice.

  title={Evaluation of approaches to generation of tissue-specific knock-in mice.},
  author={Jos{\'e} Ram{\'o}n Bayascas and Kei Sakamoto and Laura J Armit and James Simon Campbell Arthur and Dario R Alessi},
  journal={The Journal of biological chemistry},
  volume={281 39},
We explored three approaches to create tissue-specific knock-in mice by generating knock-in mice in which a substrate-docking site of the PDK1 protein kinase was ablated in Cre-expressing tissues in a way that prevented activation of one of its substrates, p70 ribosomal S6 kinase (S6K), but not another (protein kinase B (PKB)). Employing two of the approaches, termed the "heterozygous" and "minigene" methods, we generated mice in which Cre-expressing skeletal and cardiac muscle produced the… CONTINUE READING


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