BACKGROUND Medicinal plants contain large amount of natural antioxidant compounds, which enable them to neutralize free radicals. Asteraceae family is important source of phytochemical compounds. OBJECTIVE The objective of this research is to find out the antioxidant potential and radical scavenging from leaf and flowers of five species (Artemisia absinthium L., Arctium lappa L., Silybum marianum L., Centaurea cyanus L. and Echinops ritro L.) of the Asteraceae family. A similar research study has not already been done. Therefore, the findings of this research are novelty. METHODS The plant extract was obtained by absolute extraction method was investigated for total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), chain breaking activity (CBA). Thiobarbioturic acid (TBA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), IC50, nitric oxide (NO), hydrogen peroxide (H2O2), superoxide (O2-) radical scavenging and ferric reducing antioxidant power (FRAP) were, also, measured. RESULTS The results showed the highest values for the TPC, TBA, FRAP and DPPH radical scavenging related to A. absinthium. While, A. lappa extract showed the maximum content for TFC, TAC, CBA and O2- radical scavenging. In addition our results were indicated that E. ritro is a good source of NO-2 and H2O2 radical scavenging activity (P<0.05). Our findings suggest that the flower is more successful than leaf. CONCLUSION Artemisia absinthium, A. lappa and E. ritro can use as easily accessible source of natural antioxidants and as a possible food supplement or in pharmaceutical industry.