Evaluation of antigen-coating procedures of enzyme-linked immunosorbent assay method for detection of trypanosomal antibodies.

@article{Rebeski2000EvaluationOA,
  title={Evaluation of antigen-coating procedures of enzyme-linked immunosorbent assay method for detection of trypanosomal antibodies.},
  author={Dierk E. Rebeski and Eva M. Winger and M M Robinson and Cornelia Gabler and Ronald H. Dwinger and John Crowther},
  journal={Veterinary parasitology},
  year={2000},
  volume={90 1-2},
  pages={1-13}
}
Research was undertaken to improve the antigen-coating step of indirect enzyme-linked immunosorbent assay (ELISA) method through the use of polystyrene 96-well plates precoated with antigenically stabile crude trypanosomal antigens. The plates were precoated with antigens, air dried and sealed before being packed in plastic bags with silica gel desiccant packets. Such plates stored at +4 and +37 degrees C provided an assay performance, which was superior to that of plates freshly coated with… CONTINUE READING