Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs.

@article{Knittel1998EvaluationOA,
  title={Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs.},
  author={Jeffrey P. Knittel and Dianna M Jordan and Kent J. Schwartz and Bruce H. Janke and Michael B. Roof and Steven McOrist and Delbert L Harris},
  journal={American journal of veterinary research},
  year={1998},
  volume={59 6},
  pages={722-6}
}
OBJECTIVE To evaluate polymerase chain reaction (PCR) for detection of Lawsonia intracellularis DNA in feces and an indirect fluorescent antibody test (IFAT) for detecting serum IgG antibodies in pigs exposed to L intracellularis. ANIMALS 15 seven-week-old pigs and 42 three-week-old pigs. PROCEDURE During 3 experiments, 23 pigs were inoculated with a pure culture of L intracellularis, 31 pigs served as noninoculated controls, and 3 pigs were used as sentinels. Fecal shedding of L… CONTINUE READING

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