Evaluation of androgen action on fibroblast growth by flow cytometry.


The action of androgen (dihydrotestosterone-DHT-) on fibroblast growth was evaluated by 3H thymidine incorporation in DNA, by DNA assay using 3-5 diaminobenzoic acid fixation, and by a more sophisticated technique: flow cytometry. Cell DNA and proteins were stained with propidium iodide and fluorescein isothyocyanate, respectively. We did not observe any… (More)


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