Evaluation of an automated enzyme-linked fluorescent assay for thyroxine measurement in cat and dog sera

  title={Evaluation of an automated enzyme-linked fluorescent assay for thyroxine measurement in cat and dog sera},
  author={R W Anderson and Ralf S. Mueller and Sven Reese and Astrid Wehner},
  journal={Journal of Veterinary Diagnostic Investigation},
  pages={278 - 286}
Measurement of total thyroxine (T4) is the first testing step in the work-up of thyroid disease in small animals. We evaluated an enzyme-linked fluorescent assay (ELFA) as an in-house method to measure T4 in cats and dogs. We compared the T4 concentration in sera of 122 cats and 176 dogs measured by the ELFA with an enzyme immunoassay (EIA) to assess the concordance of the 2 methods. Bias of the ELFA in cats was −11.4% and in dogs 1.4%. Using Bland–Altman plots, limits of agreement were −81.5… 
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  • Medicine, Biology
    Journal of veterinary science
  • 2006
It was considered that measurement of serum tT4 and fT4 concentrations from 11 : 00 to 14 : 00 might more easily diagnose the canine hypothyroidism in practice.