Evaluation of a solid-phase monoclonal antibody-based enzyme immunoassay for insulin in human serum.

@article{Storch1989EvaluationOA,
  title={Evaluation of a solid-phase monoclonal antibody-based enzyme immunoassay for insulin in human serum.},
  author={Martin Storch and Athanasios Alexopoulos and L. Kerp},
  journal={Journal of immunological methods},
  year={1989},
  volume={119 1},
  pages={53-7}
}
A 'sandwich-type' enzyme immunoassay for the measurement of serum insulin is described in which a monoclonal antibody-alkaline phosphatase conjugate and an antibody-immobilized polystyrene solid phase are used. Serum samples of 50 microliters can be analyzed and the enzyme immunoassay is as sensitive as the conventional radioimmunoassay for insulin. The results obtained with ELISA correlate well with those of the radioimmunoassay (r = 0.9844) and the between-assay and within-assay coefficients… CONTINUE READING