Evaluation of a real-time PCR assay for the detection of the Klebsiella pneumoniae carbapenemase genes in microbiological samples in comparison with the modified Hodge test.

@article{Raghunathan2011EvaluationOA,
  title={Evaluation of a real-time PCR assay for the detection of the Klebsiella pneumoniae carbapenemase genes in microbiological samples in comparison with the modified Hodge test.},
  author={Aditya Raghunathan and Linoj P. Samuel and Robert J. Tibbetts},
  journal={American journal of clinical pathology},
  year={2011},
  volume={135 4},
  pages={
          566-71
        }
}
Transfer of the bla(KPC) genes encoding the Klebsiella pneumoniae carbapenemase (KPC) are increasingly responsible for emerging carbapenem resistance. The modified Hodge test (MHT) is recommended for the detection of KPC. We compared MHT with a real-time polymerase chain reaction (PCR) assay targeting common subtypes of bla(KPC), using previously described forward and reverse primer sequences. The PCR product was detected using SYBR Green (Applied Biosystems, Foster City, CA) and confirmed by… 
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Citation Type

A rapid low-cost real-time PCR for the detection of klebsiella pneumonia carbapenemase genes
TLDR
The real-time PCR assay could rapidly and accurately detect KPCs -harboring strains with high analytical sensitivity and specificity.
Evaluation of a real-time PCR assay for the detection of the Klebsiella pneumoniae carbapenemase gene (blaKPC) in enterobacteriaceae isolates from clinical samples in Menoufia University hospitals, Egypt
TLDR
A rapid method for detection of Klebsiella pneumonia carbapenemases genes (blaKPC) in enterobacteriaceae isolates from clinical samples by using real time PCR and comparison of this method with ordinary phenotypic methods is evaluated.
Current methods for the identification of carbapenemases
TLDR
A summary of the available methods for carbapenemase detection is gathered and the strengths and weaknesses of each method are described.
blaKPC gene Detection in Clinical Isolates of Carbapenem Resistant Enterobacteriaceae in a Tertiary Care Hospital.
TLDR
The present study focused on determining the antibiotic resistance pattern and prevalence of bla KPC gene coding for KPC in carbapenem resistant Enterobacteriaceae and found that blaKPC gene was detected in more than half of the isolates tested.
A comparative Study between Different Laboratory Tests for Detection of Carbapenem Resistance in Klebsiella Pneumoniae
TLDR
Resistance of K.pneumoniae to carbapenems was detected in 26% of isolates and the disc diffusion susceptibility did not report major errors in comparison to E test for ertapenem.
High prevalence of Klebsiella pneumoniae carbapenemase-mediated resistance in K. pneumoniae isolates from Egypt.
  • L. Metwally, N. Gomaa, M. Attallah, N. Kamel
  • Medicine, Biology
    Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit
  • 2013
TLDR
Real-time PCR assay was used in a tertiary care hospital in Egypt to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test to report high prevalence of ertAPenem nonsusceptibility.
KPC: an important mechanism of resistance in K. pneumoniae isolates from intensive care units in the Midwest region of Brazil.
TLDR
The presence of carbapenemase-producing K. pneumoniae in intensive care units (ICU) of three major Mato Grosso do Sul hospitals located in the Midwest region of Brazil is described, providing key national epidemiology data.
Emergence of carbapenemase-producing urinary isolates at a tertiary care hospital in Dhaka, Bangladesh
Antibiogram and Genetic Characterization of Carbapenem-Resistant Gram-Negative Pathogens Incriminated in Healthcare-Associated Infections
TLDR
The accurate identification of carbapenem-resistant bacterial pathogens is pivotal for the treatment of patients, in addition to propelling appropriate contamination control measures to restrain the fast spread of such pathogens.
β-lactamase-producing Gram-negative bacteria in an intensive care unit in southern Brazil
TLDR
The lowest genetic diversity, determined by ERIC-PCR, was observed among the KPC-producing K. pneumoniae isolates and OXA-producing Acinetobacter spp.
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References

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