Evaluation of a prototype Amplicor PCR assay for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults infected with HIV-1 subtypes A, C and D.

@article{Lyamuya2000EvaluationOA,
  title={Evaluation of a prototype Amplicor PCR assay for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults infected with HIV-1 subtypes A, C and D.},
  author={Eligius F. Lyamuya and E Olausson-Hansson and Jan Albert and Fred S Mhalu and Gunnel Biberfeld},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2000},
  volume={17 1},
  pages={57-63}
}
BACKGROUND In previous evaluations, the standard Amplicor HIV-1 DNA PCR test (Roche Diagnostic Systems) has been reported to have low sensitivity for the detection of some non-B HIV-1 subtypes. It has therefore become necessary to determine the performance of commercially available as well as prototype HIV-1 PCR assays for HIV-1 DNA detection in samples from various geographical settings, in order to assess their ability to detect the different HIV-1 genotypes. OBJECTIVES To determine the… CONTINUE READING