Evaluation of a polymerase chain reaction-based assay for diagnosis of Wuchereria bancrofti infection.

Abstract

To assess the utility of a polymerase chain reaction (PCR)-based method for diagnosis of Wuchereria bancrofti infection, blood, plasma, and paraffin-embedded tissue samples were tested using a PCR-based assay that detects a W. bancrofti-specific repetitive DNA sequence. The assay was positive in 100 microL of blood from 40 of 42 microfilaria-positive subjects, the 2 subjects with negative assays having microfilarial counts of 1. Samples from 127 uninfected subjects were PCR-negative. The assay was also positive in 7 of 10 daytime samples in regions where infection is nocturnally periodic; PCR amplification from paraffin-embedded sections established the diagnosis of W. bancrofti infection in another 2 cases. A microtiter ELISA plate-based method was developed for rapid evaluation of large numbers of samples. These results suggest that this PCR-based assay will be useful in diagnosis of W. bancrofti infection in a variety of clinical settings.

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@article{McCarthy1996EvaluationOA, title={Evaluation of a polymerase chain reaction-based assay for diagnosis of Wuchereria bancrofti infection.}, author={James S McCarthy and Masachika Zhong and Ramya Gopinath and Eric A . Ottesen and Steven A. Williams and T B Nutman}, journal={The Journal of infectious diseases}, year={1996}, volume={173 6}, pages={1510-4} }