Evaluation of a polymerase chain reaction-electrospray ionization time-of-flight mass spectrometry for the detection and subtyping of influenza viruses in respiratory specimens.

@article{Mengelle2013EvaluationOA,
  title={Evaluation of a polymerase chain reaction-electrospray ionization time-of-flight mass spectrometry for the detection and subtyping of influenza viruses in respiratory specimens.},
  author={Catherine Mengelle and Jean Michel Mansuy and Isabelle Ferreira da Silva and Jean-Luc Gu{\'e}rin and Jacques Izopet},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2013},
  volume={57 3},
  pages={222-6}
}
BACKGROUND PCR coupled to electrospray ionization mass spectrometry technology (PCR/ESI-TOF-MS) (PLEX-ID system, Abbott Ibis Biosciences) was developed to characterize microbial pathogens. OBJECTIVES To evaluate the performance of the PLEX-ID flu detection™ kit for detecting Influenza viruses by comparison with the multiplex RespiFinder(®) Kit (PathoFinder). STUDY DESIGN Acute-phase respiratory samples (n=293) were analysed for this purpose. A subpopulation of influenza type A positive… CONTINUE READING