Evaluation of a new selective enrichment broth for detection of group B streptococci in pregnant women.

Abstract

Studies at two Brown Medical School-affiliated hospitals were undertaken to evaluate a new selective broth medium (GBS broth) and to compare it to the LIM broth currently used to culture for group B streptococci. Beta-hemolytic group B streptococci produce a carotenoid pigment that turns GBS broth an orange color. From a total of 580 pregnant women, duplicate vaginal-rectal swabs were collected at 35 to 37 weeks of gestation and cultured for group B streptococci, using either LIM broth (a selective broth containing antibiotics) or GBS broth for enrichment. Specimens were either transported to the laboratory or immediately placed in the respective enrichment broths and delivered to the laboratory. GBS broth medium had sensitivity, specificity, and positive and negative predictive values of 87.8, 100, 100, and 95.1% when planted in the laboratory and 90.3, 100, 100 and 97.6%, respectively, when inoculated at bedside. Use of GBS broth would satisfy Centers for Disease Control and Prevention requirements and would provide faster, more-sensitive, and cost-effective detection of group B streptococci in pregnant women.

2 Figures and Tables

Statistics

0100200300'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

723 Citations

Semantic Scholar estimates that this publication has 723 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Heelan2005EvaluationOA, title={Evaluation of a new selective enrichment broth for detection of group B streptococci in pregnant women.}, author={Judith S Heelan and Judith Struminsky and Patricia Lauro and Chris Sung}, journal={Journal of clinical microbiology}, year={2005}, volume={43 2}, pages={896-7} }