Evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in Italy.

@article{Calderaro2004EvaluationOA,
  title={Evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in Italy.},
  author={Adriana Calderaro and Giovanna Piccolo and Claudia Zuelli and Lucia Galati and Leonardo Ricci and Francesca Perandin and Maria Lucia Dell'anna and Maria Cristina Arcangeletti and Maria Cristina Medici and Nino Manca and G. Dettori and Carlo Chezzi},
  journal={The new microbiologica},
  year={2004},
  volume={27 2},
  pages={163-71}
}
A new molecular diagnostic method "Malaria-IBRIDOGEN" (Amplimedical S.p.A.--Bioline Division, Turin, Italy) based on a plate-hybridization assay for the simultaneous detection and identification of human malaria parasites was evaluated in this study. A target DNA sequence of the plasmodial 18S ribosomal RNA gene was amplified by polymerase chain reaction (PCR) and hybridized in microtiter wells with five biotinylated probes each specific for Plasmodium falciparum, P. vivax, P. malariae, P… CONTINUE READING