Evaluation of a nested PCR targeting IS6110 of Mycobacterium tuberculosis for detection of the organism in the leukocyte fraction of blood samples.

@article{Nandagopal2010EvaluationOA,
  title={Evaluation of a nested PCR targeting IS6110 of Mycobacterium tuberculosis for detection of the organism in the leukocyte fraction of blood samples.},
  author={Balaji Nandagopal and Sathish Sankar and Karthikeyan Lingesan and Kumarasekharan Chandrasekharan Appu and Gopalan Sridharan and Ajay Gopinathan},
  journal={Indian journal of medical microbiology},
  year={2010},
  volume={28 3},
  pages={227-32}
}
PURPOSE Tuberculosis poses a serious health problem in resource-poor settings such as India. Polymerase chain reaction (PCR) is presently seen as a promising alternative to conventional smear microscopy and culture techniques. Undiagnosed fever is a condition where the aetiology could include tuberculosis in a significant percentage. This paper evaluates a nested PCR (nPCR) using Hotstar Taq for the detection of M. tuberculosis in patients with febrile illness using insertion element, IS6110 as… CONTINUE READING