Evaluation of a manual DNA extraction protocol and an isothermal amplification assay for detecting HIV-1 DNA from dried blood spots for use in resource-limited settings.

@article{Jordan2012EvaluationOA,
  title={Evaluation of a manual DNA extraction protocol and an isothermal amplification assay for detecting HIV-1 DNA from dried blood spots for use in resource-limited settings.},
  author={Jeanne Ann Jordan and Christine O Ibe and Miranda S Moore and Christel Host and Gary L. Simon},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2012},
  volume={54 1},
  pages={11-4}
}
BACKGROUND In resource-limited settings (RLS) dried blood spots (DBS) are collected on infants and transported through provincial laboratories to a central facility where HIV-1 DNA PCR testing is performed using specialized equipment. Implementing a simpler approach not requiring such equipment or skilled personnel could allow the more numerous provincial laboratories to offer testing, improving turn-around-time to identify and treat infected infants sooner. OBJECTIVES Assess performances of… CONTINUE READING

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