Evaluation of a line probe assay for the rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis.

@article{Ando2011EvaluationOA,
  title={Evaluation of a line probe assay for the rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis.},
  author={Hiroki Ando and Satoshi Mitarai and Yuji Kondo and Toshinori Suetake and Seiya Kato and Toru Mori and Teruo Kirikae},
  journal={Journal of medical microbiology},
  year={2011},
  volume={60 Pt 2},
  pages={
          184-8
        }
}
The aim of this study was to establish the importance of detecting fluoroquinolone (FQ) resistance in multidrug resistant (MDR) Mycobacterium tuberculosis, and to show the usefulness of a hybridization-based line probe assay (LiPA) for detecting gyrA mutations. Thirty-three MDR M. tuberculosis isolates were collected from a total of sixty MDR isolates identified in Japan over 6 months during a national surveillance study in 2002. Seventeen MDR isolates were collected by the National Center for… 
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First report of molecular detection of fluoroquinolone resistance-associated gyrA mutations in multidrug-resistant clinical Mycobacterium tuberculosis isolates in Kuwait
TLDR
Although fluoroquinolones are generally not used for chemotherapy of TB and drug susceptibility testing for second-line drugs is not carried out in Kuwait, four of 55 individual patient MDR-TB strains contained mutations in gyrA gene, and the data advocate routine drug susceptibilityTesting for this important second- line drug for proper management of M DR-TB in Kuwait.
Detection of streptomycin and quinolone resistance in Mycobacterium tuberculosis by a low-density DNA array.
Comprehensive Multicenter Evaluation of a New Line Probe Assay Kit for Identification of Mycobacterium Species and Detection of Drug-Resistant Mycobacterium tuberculosis
TLDR
The LiPA kit was able to identify mycobacterial bacilli at the species level, as well as drug-resistant phenotypes, with a high sensitivity and specificity.
Frequency and Geographic Distribution of gyrA and gyrB Mutations Associated with Fluoroquinolone Resistance in Clinical Mycobacterium Tuberculosis Isolates: A Systematic Review
TLDR
Molecular diagnostics based on detection of all gyrA mutations demonstrated to confer resistance should have broad and global utility, specifically the Genotype MTBDRsl assay, focusing on codons 88–94 should have moderate to high sensitivity in most countries.
New Insights into Fluoroquinolone Resistance in Mycobacterium tuberculosis: Functional Genetic Analysis of gyrA and gyrB Mutations
TLDR
It is indicated that certain mutations in gyrB confer fluoroquinolone resistance, but the level and pattern of resistance varies among the different mutations.
Evaluation of a novel line-probe assay for genotyping-based diagnosis of Mycobacterium tuberculosis in Thailand.
  • S. Rienthong, C. Boonin, +6 authors D. Rienthong
  • Medicine, Biology
    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2015
TLDR
The novel LiPA kits have high sensitivity and specificity, and may enhance the rapid detection of first- and second-line anti-tuberculosis drug resistance, improving the selection of suitable chemotherapy agents to treat multidrug-resistant and extensively drug-resistant TB.
Clonal expansion of Mycobacterium tuberculosis isolates and coexisting drug resistance in patients newly diagnosed with pulmonary tuberculosis in Hanoi, Vietnam
TLDR
The authors' results suggest that drug-resistant strains, particularly those with INH resistance characterized by a single mutation, S315T, are spreading in Hanoi, Vietnam, and patients are not easily cured by conventional short-term treatment.
Prevalence and Risk Factors of Drug Resistant Mycobacterium Tuberculosis in a Multisite Cohort Study
TLDR
The results from this study found the overall prevalence of MDRTB was 79.7%, 51.1% and 15% in Mumbai, Chisinau and Port Elizabeth, respectively, and the burden of DRTB was high in all three sites highlighting the importance of continuous surveillance to identify DRTB, especially among patients who have been previously treated for TB.
In vitro activity of sitafloxacin against Mycobacterium tuberculosis with gyrA/B mutations isolated in Japan
TLDR
SFX had the lowest cumulative MIC and a relatively low ECOFF value against Mtb, indicating that SFX was not only more effective against gyrA‐mutant isolates, but also MDR isolates in Japan.
Phylogenetic polymorphisms in antibiotic resistance genes of the Mycobacterium tuberculosis complex.
TLDR
This study represents one of the most extensive overviews of phylogenetically informative polymorphisms in known resistance genes to date, and will serve as a resource for the design and interpretation of genotypic susceptibility assays.
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References

SHOWING 1-10 OF 21 REFERENCES
Detection of Multidrug Resistance in Mycobacterium tuberculosis
TLDR
A DNA sequencing-based method used to detect mutations in the genome of drug-resistant Mycobacterium tuberculosis is useful for rapid detection of multiple-drug-resistant M. tuberculosis and for identifying novel mutations in drug- resistant M.culosis.
Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains
TLDR
A reverse hybridization-based line probe assay with oligonucleotide probes designed to detect mutations in pncA that appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use.
Pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis isolates in Japan.
  • H. Ando, S. Mitarai, +5 authors T. Kirikae
  • Biology, Medicine
    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2010
TLDR
Thirty-six multidrug-resistant (MDR) Mycobacterium tuberculosis isolates collected in Japan were examined for pyrazinamide susceptibility andpyrazinamidase activity, and a hybridization-based line probe assay (LiPA) was used to detect pncA mutations for the rapid identification of pyraz inamide-resistant isolates.
Diagnosis of drug-resistant tuberculosis: reliability and rapidity of detection.
  • A. Van Deun, A. Martin, J. Palomino
  • Medicine
    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2010
TLDR
An overview of the most important methods for rapid drug susceptibility testing (DST) is given, discussing their merits and shortcomings, emphasising techniques that offer an alternative to the commercial systems and genotypic tests.
Drug-resistant Mycobacterium tuberculosis in Japan: a nationwide survey, 2002.
  • Medicine, Biology
    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2007
TLDR
This study showed a clear decrease in the prevalence of initial and acquired drug resistance to major anti-tuberculosis drugs in Japan between 1997 and 2002, except for multidrug resistance.
Novel Gyrase Mutations in Quinolone-Resistant and -Hypersusceptible Clinical Isolates of Mycobacterium tuberculosis: Functional Analysis of Mutant Enzymes
TLDR
Describing several multidrug-resistant clinical isolates of M. tuberculosis carrying mutations in the genes encoding the GyrA or GyrB subunits associated with quinolone resistance or hypersusceptibility found that several isolates bearing a novel combination of gyrA T80A and A90G changes were hypersuceptible to ofloxacin.
Correlation between Quinolone Susceptibility Patterns and Sequences in the A and B Subunits of DNA Gyrase in Mycobacteria
TLDR
Peptide sequences of the QRDR of GyrA were identical in all the species, except for the amino acid at position 83, suggesting that this amino acid is involved in the observed differences of quinolone susceptibility within theMycobacterium genus.
Empirical treatment with a fluoroquinolone delays the treatment for tuberculosis and is associated with a poor prognosis in endemic areas
TLDR
With a 34 day delay in antituberculous treatment and more frequent coexistence of underlying disease and hypoalbuminaemia, empirical fluoroquinolone treatment was associated with a poor outcome.
Multidrug-resistant tuberculosis: epidemiology, risk factors and case finding.
  • J. Caminero
  • Medicine
    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2010
TLDR
The recognition of factors leading to the epidemic in some regions and the identification of populations at risk will assist in focusing case-finding efforts, and patients previously treated for TB and the other risk factors analysed in this article should be prioritised in case finding.
Impact of fluoroquinolones on the diagnosis of pulmonary tuberculosis initially treated as bacterial pneumonia.
  • Y. Yoon, H. J. Lee, +5 authors J. Yim
  • Medicine, Biology
    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
  • 2005
TLDR
Delays in the initiation of anti-tuberculosis treatment is possible in patients administered fluoroquinolone and initially misdiagnosed as having bacterial pneumonia.
...
1
2
3
...