Evaluation of a kinetic method for prostatic acid phosphatase with use of self-indicating substrate, 2,6-dichloro-4-nitrophenyl phosphate.

@article{Valcour1989EvaluationOA,
  title={Evaluation of a kinetic method for prostatic acid phosphatase with use of self-indicating substrate, 2,6-dichloro-4-nitrophenyl phosphate.},
  author={A. Valcour and G. Bowers and R. Mccomb},
  journal={Clinical chemistry},
  year={1989},
  volume={35 6},
  pages={
          939-45
        }
}
  • A. Valcour, G. Bowers, R. Mccomb
  • Published 1989
  • Chemistry, Medicine
  • Clinical chemistry
  • The purity, spectral characteristics, and rate of nonenzymatic hydrolysis of 2,6-dichloro-4-nitrophenyl phosphate (DCNPP) were determined. Rates of DCNPP hydrolysis by prostatic acid phosphatase (PAP) and erythrocytic acid phosphatase (EAP) (both EC 3.1.3.2) were measured in the absence and in the presence of various alcohols. 1.5-Pentanediol was the most effective transphosphorylation agent for specifically enhancing the activity of PAP. 1,4-Butanediol also enhanced PAP activity but markedly… CONTINUE READING
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