Evaluation of a kinetic method for prostatic acid phosphatase with use of self-indicating substrate, 2,6-dichloro-4-nitrophenyl phosphate.

@article{Valcour1989EvaluationOA,
  title={Evaluation of a kinetic method for prostatic acid phosphatase with use of self-indicating substrate, 2,6-dichloro-4-nitrophenyl phosphate.},
  author={A. Valcour and G. Bowers and R. Mccomb},
  journal={Clinical chemistry},
  year={1989},
  volume={35 6},
  pages={
          939-45
        }
}
  • A. Valcour, G. Bowers, R. Mccomb
  • Published 1989
  • Chemistry, Medicine
  • Clinical chemistry
    • The purity, spectral characteristics, and rate of nonenzymatic hydrolysis of 2,6-dichloro-4-nitrophenyl phosphate (DCNPP) were determined. Rates of DCNPP hydrolysis by prostatic acid phosphatase (PAP) and erythrocytic acid phosphatase (EAP) (both EC 3.1.3.2) were measured in the absence and in the presence of various alcohols. 1.5-Pentanediol was the most effective transphosphorylation agent for specifically enhancing the activity of PAP. 1,4-Butanediol also enhanced PAP activity but markedly… CONTINUE READING
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    References

    SHOWING 1-10 OF 27 REFERENCES
    Determination of acid phosphatase in biological fluids using a new substrate, 2,6-dichloro-4-nitrophenyl phosphate.
    • 10
    • Highly Influential
    Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum.
    • 238
    • PDF
    Studies on human prostatic acid phosphatase. III. The effects of alcohols on human erythrocyte acid phosphatase and on human prostatic acid phosphatase.
    • 2
    Sodium thymolphthalein monophosphate: a new acid phosphatase substrate with greater specificity for the prostatic enzyme in serum.
    • 230
    • PDF
    Kinetic analysis of chemical reactions coupled to an enzymic step. Application to acid phosphatase assay with Fast Red.
    • 9
    Reaction of plasma albumin with I-naphthyl N-methylcarbamate and certain other esters.
    • 93
    Aktivierung der sauren Prostataphosphatase durch 1-Pentanol
    • 4
    • PDF
    Total, tartrate-resistant, and tartrate-inhibited acid phosphatases in serum: biological variations and reference limits.
    • 31
    • PDF
    High-purity 4-nitrophenol: purification, characterization, and specifications for use as a spectrophotometric reference material.
    • 73
    • PDF
    An optimized continuous-monitoring procedure for semiautomated determination of serum acid phosphatase activity.
    • 35
    • PDF