Evaluation of a direct colorimetric assay for rapid detection of rifampicin resistant Mycobacterium tuberculosis

@inproceedings{Woldemeskel2005EvaluationOA,
  title={Evaluation of a direct colorimetric assay for rapid detection of rifampicin resistant Mycobacterium tuberculosis},
  author={Dawit Woldemeskel and Getahun Abate and Mekuria Lakew and S. Belay Goshu and Alemayehu Selassie and Hakan Miorner and Abraham Aseffa},
  year={2005}
}
Background: Susceptibility test is a useful tool for selecting and modifying appropriate treatment for tuberculosis. Objective: To evaluate a direct colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) for a rapid detection of rifampicin resistance. Methods: Sputum was inoculated directly into 7H9 broth supplemented with PANTA (antibiotic mixture) and oleic acid albumin dextrose catalase (OADC) to which MTT was added after 1-3 weeks. Bacterial growth is… CONTINUE READING

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PANTA is a standard antibiotic cocktail that contains Polymyxin B , Amphotericin B , Nalidixic acid , Trimethoprim and Azlocillin .
PANTA is a standard antibiotic cocktail that contains Polymyxin B , Amphotericin B , Nalidixic acid , Trimethoprim and Azlocillin .
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