Evaluation of a cleavable stable isotope labeled synthetic peptide for absolute protein quantification using LC-MS/MS.

@article{Barnidge2004EvaluationOA,
  title={Evaluation of a cleavable stable isotope labeled synthetic peptide for absolute protein quantification using LC-MS/MS.},
  author={David R. Barnidge and Gregory Daniel Hall and Jeanne L Stocker and David C Muddiman},
  journal={Journal of proteome research},
  year={2004},
  volume={3 3},
  pages={
          658-61
        }
}
Protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS) has the potential to provide the absolute concentration of a specific protein, or multiple proteins, in complex mixtures. However, PC-IDMS differs from standard IDMS since the internal standard is a different molecule than the analyte at the start of the experiment, more specifically, the internal standard is a peptide and the analyte is a protein prior to cleavage. It is not until after the cleavage process that the… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 20 CITATIONS

Clinical mass spectrometry proteomics (cMSP) for medical laboratory: What does the future hold?

  • Clinica chimica acta; international journal of clinical chemistry
  • 2017
VIEW 2 EXCERPTS
CITES BACKGROUND