Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia.

@article{Cienfuegos2011EvaluationOA,
  title={Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia.},
  author={Astrid Vanessa Cienfuegos and Doris A Rosero and Nelson J Naranjo and Shirley Luckhart and Jan E Conn and Margarita M Correa},
  journal={Acta tropica},
  year={2011},
  volume={118 2},
  pages={128-35}
}
Anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. The aim of this work was to evaluate the applicability of a previously developed PCR-RFLP-ITS2 assay for accurate discrimination of anophelines in twelve localities spanning three Colombian malaria epidemiological regions: Atlantic Coast, Pacific Coast, and Uraba-Bajo Cauca-Alto Sinu region. The evaluation of… CONTINUE READING

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