Evaluation of Toluidine Blue O Staining for the Diagnosis of Pneumocystis jiroveci in Expectorated Sputum Sample and Bronchoalveolar Lavage from HIV-infected Patients in a Tertiary Care Referral Center in Ethiopia

@article{Aderaye2007EvaluationOT,
  title={Evaluation of Toluidine Blue O Staining for the Diagnosis of Pneumocystis jiroveci in Expectorated Sputum Sample and Bronchoalveolar Lavage from HIV-infected Patients in a Tertiary Care Referral Center in Ethiopia},
  author={Getachew Aderaye and Yimtubezenash Woldeamanuel and Daniel Asrat and Marianne Lebbad and Jessica Beser and Alemitu Worku and Val Fern{\'a}ndez and Lars Lindquist},
  journal={Infection},
  year={2007},
  volume={36},
  pages={237-243}
}
Pneumocystis pneumonia (PCP) is becoming increasingly recognized in sub-Saharan Africa. The currently recommended diagnostic methods using induced sputum (IS) and bronchoalveolar lavage (BAL) are neither technically feasible nor affordable for a wider clinical use in developing countries. Therefore, there is a need for a simple and affordable diagnostic test. The yield of Toluidine Blue O (TBO) stain, immunofluorescence (IF), and polymerase chain reaction (PCR) for the diagnosis of Pneumocystis… CONTINUE READING

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