Evaluation of Three RT-qPCR-Based miRNA Detection Methods Using Seven Rice miRNAs

@article{Mou2013EvaluationOT,
  title={Evaluation of Three RT-qPCR-Based miRNA Detection Methods Using Seven Rice miRNAs},
  author={Guiping Mou and Kang Wang and Donglin Xu and Guohui Zhou},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2013},
  volume={77},
  pages={1349 - 1353}
}
Three frequently-used reverse transcription-quantitative polymerase chain reaction (RT-qPCR)-based miRNA detection methods, stem-loop RT-qPCR, poly(A)-tailing RT-qPCR, and miQPCR, were evaluated using seven selected rice miRNAs. The results revealed that miRNA abundance and sequence characteristics can affect capability of detection. The stem-loop amplification technique detected highly and moderately abundant miRNAs. The poly(A)-tailing method detected both highly abundant and sparsely present… 
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