Evaluation of Temperature Gradient Capillary Electrophoresis for Detection of the Factor V Leiden Mutation

@article{Murphy2003EvaluationOT,
  title={Evaluation of Temperature Gradient Capillary Electrophoresis for Detection of the Factor V Leiden Mutation},
  author={Dr Kathleen M. Murphy and Michael J. Hafez and Juliet Philips and Kellie Yarnell and Kevin R. Gutshall and K J Borgonjen-van den Berg},
  journal={Molecular Diagnosis},
  year={2003},
  volume={7},
  pages={35-40}
}
Aim: The Factor V Leiden mutation (G1691A) is a clinically important polymorphism that results in an increased risk of thrombosis. The goal of this study was to compare a temperature gradient capillary electrophoresis (TGCE) platform for the detection of Factor V gene mutations to a conventional restriction fragment length polymorphism (RFLP) assay. Methods: Three hundred and four samples were analyzed by both TGCE and a common clinical Mnl I/RFLP assay. Concordance of results between the two… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 18 references

Ethnic distribution of Factor V cysteine metabolism

  • PM Ridker, JP Miletich, CH Hennekens
  • Clin Chem
  • 2000
1 Excerpt

Non - PCRdependent detection of the Factor V Leiden mutation from genomic DNA using a homogeneous invader microtiter plateThe authors would like to thank Dr James Eshleman for helpful technical assay

  • A Kowalski, D Radu, B Gold
  • Mol Diagn
  • 1999

Similar Papers

Loading similar papers…