Evaluation of MPN method combined with PCR procedure for detection and enumeration of Vibrio parahaemolyticus in seafood.

@article{Miwa2003EvaluationOM,
  title={Evaluation of MPN method combined with PCR procedure for detection and enumeration of Vibrio parahaemolyticus in seafood.},
  author={Norinaga Miwa and Tomohiro Nishio and Yono Arita and Fumihiko Kawamori and Takashi Masuda and Masato Akiyama},
  journal={Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan},
  year={2003},
  volume={44 6},
  pages={289-93}
}
Vibrio parahaemolyticus densities in spiked and naturally contaminated seafood samples were enumerated by the MPN method combined with a PCR procedure (MPN-PCR method) targeting the species-specific thermolabile hemolysin gene (tlh), and by the MPN method using subcultivation of alkaline-peptone-water (APW) enrichment culture on thiosulfate-citrate-bile… CONTINUE READING