Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques

@inproceedings{Elkhawagah2014EvaluationOI,
  title={Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques},
  author={A. R. Elkhawagah and Valentina Longobardi and Bianca Gasparrini and Gamal Abd El-Raheim Sosa and Giuseppe Bifulco and Mahmoud E. A. Abou-El-Roos and Abeer E. Abd El-Ghafar and G. Camapnile},
  year={2014}
}
This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm cells were incubated in Tyrode albumin lactate pyruvate medium (TALP) in absence of capacitating agents (control) and in presence of 10 µg/ml heparin for 2 and 4 h. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylcholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein… CONTINUE READING

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This work was supported by POR CAMPANIA FSE 2007 / 2013 . “ Sviluppo di Reti di Eccellenza tra Università , Centri di Ricerca , Imprese ”

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