Evaluation of Generation 2 and 3 Poly(Propylenimine) Dendrimers for the Potential Cellular Delivery of Antisense Oligonucleotides Targeting the Epidermal Growth Factor Receptor

@article{Hollins2004EvaluationOG,
  title={Evaluation of Generation 2 and 3 Poly(Propylenimine) Dendrimers for the Potential Cellular Delivery of Antisense Oligonucleotides Targeting the Epidermal Growth Factor Receptor},
  author={Andrew J. Hollins and Mustapha Benboubetra and Yadollah Omidi and Bernd H. Zinselmeyer and Andreas G. Schatzlein and Ijeoma F. Uchegbu and Saghir Akhtar},
  journal={Pharmaceutical Research},
  year={2004},
  volume={21},
  pages={458-466}
}
Purpose. To evaluate low generation, G2 and G3, poly(propylenimine) dendrimers for the potential cellular delivery of antisense oligonucleotides (ODNs) targeting the epidermal growth factor receptor (EGFR) in A431 epidermoid carcinoma cells. Methods. Cell cytotoxicity of the dendrimers was evaluated using trypan blue exclusion assays. Cellular uptake studies of fluorescently labeled ODNs were performed using fluorescence-activated cell sorting analysis. Intracellular fate of dendrimer-delivered… CONTINUE READING