Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana

  title={Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana},
  author={Gordana Marjanac and Annelies De Paepe and Ingrid Peck and Anni Jacobs and Sylvie De Buck and Anna Depicker},
  journal={Transgenic Research},
The ability of the CRE recombinase to catalyze excision of a DNA fragment flanked by directly repeated lox sites has been exploited to modify gene expression and proved to function well in particular case studies. However, very often variability in CRE expression and differences in efficiency of CRE-mediated recombination are observed. Here, various approaches were investigated to reproducibly obtain optimal CRE activity. CRE recombination was analyzed either by transforming the CRE T-DNA into… CONTINUE READING