Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, Chlorobenzoylureido Cephalosporin, BL-P1654, and Carbenicillin on Pseudomonas aeruginosa

@article{Ellis1976EvaluationOA,
  title={Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, Chlorobenzoylureido Cephalosporin, BL-P1654, and Carbenicillin on Pseudomonas aeruginosa},
  author={Lee F. Ellis and David K. Herron and David A. Preston and Linda K. Simmons and R A Schlegel},
  journal={Antimicrobial Agents and Chemotherapy},
  year={1976},
  volume={9},
  pages={334 - 342}
}
The response of Pseudomonas aeruginosa to carbenicillin, BL-P1654, and two cephalosporins (112384 and 112883) was evaluated by minimal inhibitory concentration determinations, [14C]leucine uptake studies, morphological studies of colonial growth, and mouse intraperitoneal inoculations. Spheroplast formation and bacterial lysis were not the early response; instead, cell division was inhibited and long filaments were formed. Spheroplast formation and bacterial lysis were not observed in the first… 
Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, BL-P1654, and Carbenicillin on Escherichia coli
Early response of Escherichia coli to minimal inhibitory concentrations of 112883, BL-P1654, and carbenicillin was determined by [14C]leucine uptake and scanning electron microscopy morphology
Comparative Effects of Amoxycillin and Ampicillin on the Morphology of Escherichia coli In Vivo and Correlation with Activity
TLDR
An experimental mouse intraperitoneal infection due to Escherichia coli was treated with subcutaneous amoxycillin or ampicillin, and it was concluded that the superior therapeutic activity of amoxy councillors is due to its greater bacteriolytic activity in vivo.
Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action
TLDR
A critical look at the examination of treated bacteria for changes to their morphology and ultrastructure and explains how changes that are induced by multiple antibacterial classes can still yield useful mechanistic information.
Resistance of Pseudomonas aeruginosa PAO to nalidixic acid and low levels of beta-lactam antibiotics: mapping of chromosomal genes
TLDR
In nalB mutants, DNA replication showed wild- type sensitivity to nalidixic acid, whereas carbenicillin-induced filamentation required higher drug levels than in the wild-type strain, Thus, n alB mutations appear to decrease cell permeability to some antibiotics.
The effect of subminimal inhibitory concentrations of antimicrobial agents on three bacterial mixtures.
TLDR
3 mixtures where these bacterial interactions change the sensitivity to antimicrobials or change the proportions of each bacterial species in the mixture are found where F. nucleatum seems to protect B. endodontalis from the action of metronidazole.
THEORETICAL STUDIES OF β‐LACTAM ANTIBIOTICS *
TLDR
The number of classes of p-lactam antibiotics continues to grow and the most familiar class contains the penicillins, which are a very highly prescribed antibiotic today.

References

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Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, BL-P1654, and Carbenicillin on Escherichia coli
Early response of Escherichia coli to minimal inhibitory concentrations of 112883, BL-P1654, and carbenicillin was determined by [14C]leucine uptake and scanning electron microscopy morphology
Inhibition of Pseudomonas pseudomallei by carbenicillin, in vitro.
  • M. Franklin
  • Biology
    Canadian journal of microbiology
  • 1974
TLDR
The activity of carbenicillin on 20 strains of Pseudomonas pseudomallei was determined by the agar-diffusion and tube-dilution methods and early logarithmic phase cells were more sensitive to CB than stationary phase cells.
BL-P1654, Ticarcillin, and Carbenicillin: In Vitro Comparison Alone and in Combination with Gentamicin Against Pseudomonas aeruginosa
TLDR
Adding gentamicin enhanced the inhibitory and bactericidal activity of all three penicillin derivatives for the majority of isolates, however, inhibition of isolate highly resistant to Gentamicin was not improved by combining the semisynthetic penicillins with gentamic in.
Effects of Sublethal Concentrations of Benzylpenicillin on Pseudomonas aeruginosa
TLDR
Pseudomonas aeruginosa produces a low basal level of β-lactamase, which is analogous to the behavior of gram-positive bacteria rather than to that of Enterobacteriaceae, and the 10-fold lower resistance to carbenicillin than to benzylpenicillin can be explained by the inability of the inducible β-methioninease to hydrolyze carbeniillin.
Morphological Alterations of Pseudomonas aeruginosa by Ticarcillin: a Scanning Electron Microscope Study
Pseudomonas aeruginosa was exposed to 0.1, 1.0, 10, 100, and 1,000 times the minimal inhibitory concentration of ticarcillin in vitro and subsequently examined with the scanning electron microscope.
Comparison of the Responses of Escherichia coli and Proteus mirabilis to Seven β-Lactam Antibiotics
TLDR
The profiles of turbidimetric and morphologic response of three strains of enterobacteria producing respectively fast, slow, and no f-lactamase have been compared for seven B3-l lactam antibiotics, offering a different, and perhaps more meaningful, picture of the relative activity of 3- lactam agents than do conventional determinations of minimal inhibitory concentration.
Murein and the Outer Penetration Barrier of Escherichia coli K-12, Proteus mirabilis, and Pseudomonas aeruginosa
TLDR
Results indicate that the murein sacculus either is a part of the penetration barrier or is responsible for holding the structure of the outer membrane together in an ampicillin-resistant mutant of Escherichia coli K-12.
dd-Carboxypeptidase and Peptidoglycan Transpeptidase from Pseudomonas aeruginosa
TLDR
Peptidoglycan transpeptidase from Escherichia coli, which is sensitive to inhibition by penicillins and cephalosporins, is detected in isolated membranes of Pseudomonas aeruginosa.
Induction Kinetics of β-Lactamase Biosynthesis in Pseudomonas aeruginosa
TLDR
The induction of β-lactamase in Pseudomonas aeruginosa 1822s was studied using benzylpenicillin as inducer and the basal and the induced enzymes seem to be identical since they had the same substrate profile, electrophoretic mobility, and molecular weight.
Antibiotic-induced alterations in the surface morphology of bacterial cells: a scanning-beam electron miscroscopy study.
TLDR
The SEM was utilized to study the effects of penicillin G, cephalothin, and carbenicillin on the surface morphology of certain strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, respectively.
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