Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens.

@article{Beavis1995EvaluationOA,
  title={Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens.},
  author={K G Beavis and M B Lichty and Donald L. Jungkind and Olarae Giger},
  journal={Journal of clinical microbiology},
  year={1995},
  volume={33 10},
  pages={2582-6}
}
We evaluated the Amplicor PCR assay (Roche Molecular Systems, Branchburg, N.J.) for direct detection of Mycobacterium tuberculosis in sputum. A total of 532 specimens from 270 patients were decontaminated and stored at 4 or -75 degrees C until assayed by PCR. This assay used three-step sample preparation, biotinylated primer pairs, AmpErase, and a microtiter format for amplicon capture and detection. Amplicor PCR results were compared with clinical history, culture from a Lowenstein-Jensen… CONTINUE READING