Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in mice.

@article{Vanderheyden2006EvaluationO9,
  title={Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in mice.},
  author={J. E. Vanderheyden and Guozheng Liu and Jiang He and Bhavesh Sumantrai Patel and Jonathan K. F. Tait and Donald J. Hnatowich},
  journal={Nuclear medicine and biology},
  year={2006},
  volume={33 1},
  pages={
          135-44
        }
}
We conjugated mercaptoacetyltriglycine (MAG(3)) to rh-annexin V to permit radiolabeling with (99m)Tc in an effort to decrease the high kidney and liver accumulation observed for (99m)Tc-labeled Hynic-annexin V. The 36-kDa protein was conjugated at a 5:1 molar ratio with NHS-MAG(3) in HEPES buffer pH 7.8 at room temperature, then quenched with glycine and purified by dialysis. The biopotency of the resulting MAG(3)-annexin was similar to that of Hynic-annexin as determined by a sensitive red… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 23 CITATIONS

Similar Papers

Loading similar papers…