Evaluation of 7SL RNA gene sequences for the identification of Leishmania spp.

@article{Zelazny2005EvaluationO7,
  title={Evaluation of 7SL RNA gene sequences for the identification of Leishmania spp.},
  author={Adrian M. Zelazny and Daniel P Fedorko and Li Li and Franklin A. Neva and Steven H. Fischer},
  journal={The American journal of tropical medicine and hygiene},
  year={2005},
  volume={72 4},
  pages={415-20}
}
We evaluated the use of 7SL RNA gene sequences for the identification of Leishmania spp. A fragment (approximately 137 basepairs) of the 7SL RNA gene from 13 reference strains and 18 clinical isolates of 11 different Leishmania species was amplified and sequenced using conserved primers. Reference strains from each Leishmania spp. complex showed unique sequences. The nucleotide sequences were compared pairwise and a range of 81.0-99.3% intercomplex similarity was observed. Clinical isolates of… CONTINUE READING

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