• DOI:10.1007/s10295-016-1793-z
Corpus ID: 18901771

Evaluation of 3-hydroxypropionate biosynthesis in vitro by partial introduction of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula

@article{Ye2016EvaluationO3,
  title={Evaluation of 3-hydroxypropionate biosynthesis in vitro by partial introduction of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula},
  author={Ziling Ye and X. Li and Yongbo Cheng and Z. Liu and Gaoyi Tan and F. Zhu and Shuai Fu and Z. Deng and Tiangang Liu},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2016},
  volume={43},
  pages={1313 - 1321}
}

Ziling Ye, X. Li, +6 authors Tiangang Liu

Published 2016

Biology, Medicine

Journal of Industrial Microbiology & Biotechnology

The chemical 3-hydroxypropionate (3HP) is an important starting reagent for the commercial synthesis of specialty chemicals. In this study, a part of the 3-hydroxypropionate/4-hydroxybutyrate cycle from Metallosphaera sedula was utilized for 3HP production. To study the basic biochemistry of this pathway, an in vitro-reconstituted system was established using acetyl-CoA as the substrate for the kinetic analysis of this system. The results indicated that 3HP formation was sensitive to acetyl-CoA… CONTINUE READING

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