Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation

@article{Quail2012EvaluationAO,
  title={Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation},
  author={Michael A. Quail and Yong Gu and Harold P Swerdlow and Matthew Mayho},
  journal={ELECTROPHORESIS},
  year={2012},
  volume={33}
}
Size selection can be a critical step in preparation of next‐generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high‐throughput setting, solid‐phase reversible immobilisation beads are commonly used for size‐selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi‐automated preparative DNA… 

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