Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation

  title={Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation},
  author={Michael A. Quail and Yong Gu and Harold P Swerdlow and Matthew Mayho},
Size selection can be a critical step in preparation of next‐generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high‐throughput setting, solid‐phase reversible immobilisation beads are commonly used for size‐selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi‐automated preparative DNA… 

Best Practices for Illumina Library Preparation

These workflows are applicable to both high‐throughput and low-throughput Illumina library preparation and should help reduce bias, increase cost effectiveness, and produce high library yields.

A Microfluidic Device for Preparing Next Generation DNA Sequencing Libraries and for Automating Other Laboratory Protocols That Require One or More Column Chromatography Steps

A microfluidic device for automating laboratory protocols that require one or more column chromatography steps is described and its utility for preparing Next Generation sequencing libraries for the Illumina and Ion Torrent platforms is demonstrated.

Say Goodbye to Manual Gels : Automated DNA Size Selection Has Arrived

  • Biology, Business
  • 2012
It has been well established in the scientific literature that while next-generation sequencers have used automation to increase throughput, reduce time to results, and lower costs, there continues

Improved Protocols for Illumina Sequencing

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A tunable filter for high molecular weight DNA selection and linked-read sequencing.

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All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing

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From Benchtop to Desktop: Important Considerations when Designing Amplicon Sequencing Workflows

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Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)

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SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing

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Increased Throughput by Parallelization of Library Preparation for Massive Sequencing

An automated parallel library preparation protocol using generic carboxylic acid-coated superparamagnetic beads and polyethylene glycol precipitation as a reproducible and flexible method for DNA fragment length separation is demonstrated, which enables a considerable efficiency increase for small to midsize sequencing centers.

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

The overall performance of all three methods was equal with only minor differences, and enzymatic fragmentation showed highest consistency between three library preparations but performed slightly worse than sonication and nebulization with regard to insertions/deletions in the raw sequence reads.

Scalable Transcriptome Preparation for Massive Parallel Sequencing

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Optimal enzymes for amplifying sequencing libraries

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