Evaluation and improvement of a rapid microassay for measuring superoxide anion production by phagocytes. 1. Spectrophotometric aspects.


Phagocyte synthesis of superoxide anion can be reliably measured as superoxide dismutase-inhibitable reduction of ferricytochrome C (Cytc). The original assay involved separation of the Cytc solution from the cells before estimating the extent of reduction spectrophotometrically. More recently, a rapid microplate assay has been devised, in which the cells… (More)


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