Evaluation and comparison of the insulation efficiency of three enhancer-blocking insulators in plants

@article{Yang2010EvaluationAC,
  title={Evaluation and comparison of the insulation efficiency of three enhancer-blocking insulators in plants},
  author={Yazhou Yang and Stacy D. Singer and Zongrang Liu},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2010},
  volume={105},
  pages={405-414}
}
Enhancer-promoter interactions have the potential to compromise the precise engineering of gene function and agronomically important traits in crops; therefore, effective enhancer-blocking insulators are required in transgene constructs to block such interference. In this study, we evaluated and compared the insulation efficiency of three enhancer-blocking insulators using an enhancer activation assay in which a flower-specific AGAMOUS second intron-derived promoter (AGIP) that drives a… CONTINUE READING
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