Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.

  title={Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.},
  author={Yasuhiko Miwa and Atsuo Nakata and A. Ogiwara and Masayuki Yamamoto and Yasutaro Fujita},
  journal={Nucleic acids research},
  volume={28 5},
A global mechanism of catabolite repression of the genus Bacillus comprises negative regulation exerted through the binding of the CcpA protein to the catabolite-responsive elements (cres) of the target genes. We searched for cre sequences in the Bacillus subtilis genome using a query sequence, WTGNAANCGNWNNCW (N and W stand for any base and A or T, respectively), picking out 126 putative and known cre sequences. To examine their cre function, we integrated spac promoter (P spac )-cre-lacZ… CONTINUE READING
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